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master cycler nexus gradient 230  (Eppendorf AG)


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    Structured Review

    Eppendorf AG master cycler nexus gradient 230
    Master Cycler Nexus Gradient 230, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/master+cycler+nexus+gradient+230/Mastercycler+nexus/pm40457246-69-57-62
    Average 97 stars, based on 1263 article reviews
    master cycler nexus gradient 230 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Amplification:

    Article Title: Carbapenem-resistance in Acinetobacter baumannii : prevalence, antibiotic resistance profile and carbapenemase genes in clinical and hospital environmental strains
    Article Snippet: .. Amplification of extracted DNA templates was carried out in 20 μL solutions, comprising 4 μL of 5x Master-Mix (Genewiz, USA), 1 μL each of the two 10 μM forward and reverse primers (Genewiz, USA), and 5 μL of each DNA template made up to 20 μL with 7 μL of DNAse/RNAse-free sterile water (BioConcept, USA) using the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Acinetobacter baumannii ATCC 19606 served as the positive control, while DNAse/RNAse-free sterile water was used as a template in the negative control setup.

    Article Title: Guardians of resistance and virulence: detection of mec , femA , Van , pvl , hlg and spa genes in methicillin and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria
    Article Snippet: .. DNA templates were amplified by Polymerase Chain Reaction (PCR) in a 20μL solution comprising 10μL of 2× Master-Mix (Biolabs, England), 1μL each of 10μM forward and reverse primers (Inqaba-Biotec, South Africa), and 5μL of DNA template made up to 20μL with 3μL of DNAse/RNAse-free sterile water (BioConcept, USA) with the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Amplicons (10μL each) were run on 1.0% SafeView-Classic stained agarose gel at 80 V for 60 min, then viewed using the UV trans-illuminator E-BOX-CX5 TS imaging system (Vilber, France).

    Article Title: Carbapenem-resistance in Acinetobacter baumannii: prevalence, antibiotic resistance profile and carbapenemase genes in clinical and hospital environmental strains.
    Article Snippet: .. Amplification of extracted DNA templates was carried out in 20 μL solutions, comprising 4 μL of 5x Master-Mix (Genewiz, USA), 1 μL each of the two 10 μM forward and reverse primers (Genewiz, USA), and 5 μL of each DNA template made up to 20 μL with 7 μL of DNAse/RNAse-free sterile water (BioConcept, USA) using the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Acinetobacter baumannii ATCC 19606 served as the positive control, while DNAse/RNAse-free sterile water was used as a template in the negative control setup.

    Article Title: Genetic Diversity of Selected Decapods in Nigerian Niger Delta Rivers Using DNA Barcoding
    Article Snippet: The target region was amplified with a cytochrome oxidase primer pair using OneTaq® Quick-Load® 2X Master Mix (NEB, Catalogue No. M0486) following the conditions presented in the Table below.Details of the primers are contained in the table below. .. The reaction mixture contained 12.5μl of OneTaq 2X MasterMix with Standard Buffer (NEB, Catalogue No. M0482S), 3μl Genomic DNA (10-30ng/μl), 0.5μl Forward primer (10μM), 0.5μl Reverse primer (10μM), and 8.5μl Nuclease free water (Catalogue No. E476). https://international.neb.com/products/m0482onetaq-2x-master-mix-with-standard buffer#Product%20Information The samples were amplified using Eppendorf Master cycler Nexus gradient 230, under the following conditions: initial denaturation at 94°C for 10mins, followed by 35 cycles of denaturation at 94°C for 30sec, annealing at 50°C for 1min, and extension at 68°C for 90sec, and then a cycle of final extension at 72°C for 10mins (Setiati et al., 2023). .. After PCR amplification, 2ul of the PCR product was run on 1% agarose gel, stained with Safe View Red (5ul) and photographed using a gel documentation system (E-BOX, Vilber Lourmat, Italy) (Numbere et al., 2024).

    Sterility:

    Article Title: Carbapenem-resistance in Acinetobacter baumannii : prevalence, antibiotic resistance profile and carbapenemase genes in clinical and hospital environmental strains
    Article Snippet: .. Amplification of extracted DNA templates was carried out in 20 μL solutions, comprising 4 μL of 5x Master-Mix (Genewiz, USA), 1 μL each of the two 10 μM forward and reverse primers (Genewiz, USA), and 5 μL of each DNA template made up to 20 μL with 7 μL of DNAse/RNAse-free sterile water (BioConcept, USA) using the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Acinetobacter baumannii ATCC 19606 served as the positive control, while DNAse/RNAse-free sterile water was used as a template in the negative control setup.

    Article Title: Occurrence of extremely drug-resistant Klebsiella and multidrug-resistant Enterobacter species in chronic wound patients
    Article Snippet: .. The PCR protocols were run with the Master Cycler Nexus Gradient 230 (Eppendorf, Germany) using 20μL solution consisting of 10μL of 2× Master-Mix (Biolabs, England), 1μL of 10μM each primer, and 5μL of the DNA template made up with 2μL of DNAse/RNAse free sterile water (BioConcept, USA) in the case of Klebsiella spp., and 3μL of DNAse/RNAse free sterile water in the case of Enterobacter spp. to the final 20μL volume. ..

    Article Title: Guardians of resistance and virulence: detection of mec , femA , Van , pvl , hlg and spa genes in methicillin and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria
    Article Snippet: .. DNA templates were amplified by Polymerase Chain Reaction (PCR) in a 20μL solution comprising 10μL of 2× Master-Mix (Biolabs, England), 1μL each of 10μM forward and reverse primers (Inqaba-Biotec, South Africa), and 5μL of DNA template made up to 20μL with 3μL of DNAse/RNAse-free sterile water (BioConcept, USA) with the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Amplicons (10μL each) were run on 1.0% SafeView-Classic stained agarose gel at 80 V for 60 min, then viewed using the UV trans-illuminator E-BOX-CX5 TS imaging system (Vilber, France).

    Article Title: Carbapenem-resistance in Acinetobacter baumannii: prevalence, antibiotic resistance profile and carbapenemase genes in clinical and hospital environmental strains.
    Article Snippet: .. Amplification of extracted DNA templates was carried out in 20 μL solutions, comprising 4 μL of 5x Master-Mix (Genewiz, USA), 1 μL each of the two 10 μM forward and reverse primers (Genewiz, USA), and 5 μL of each DNA template made up to 20 μL with 7 μL of DNAse/RNAse-free sterile water (BioConcept, USA) using the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Acinetobacter baumannii ATCC 19606 served as the positive control, while DNAse/RNAse-free sterile water was used as a template in the negative control setup.

    Polymerase Chain Reaction:

    Article Title: Occurrence of extremely drug-resistant Klebsiella and multidrug-resistant Enterobacter species in chronic wound patients
    Article Snippet: .. The PCR protocols were run with the Master Cycler Nexus Gradient 230 (Eppendorf, Germany) using 20μL solution consisting of 10μL of 2× Master-Mix (Biolabs, England), 1μL of 10μM each primer, and 5μL of the DNA template made up with 2μL of DNAse/RNAse free sterile water (BioConcept, USA) in the case of Klebsiella spp., and 3μL of DNAse/RNAse free sterile water in the case of Enterobacter spp. to the final 20μL volume. ..

    Article Title: Guardians of resistance and virulence: detection of mec , femA , Van , pvl , hlg and spa genes in methicillin and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria
    Article Snippet: .. DNA templates were amplified by Polymerase Chain Reaction (PCR) in a 20μL solution comprising 10μL of 2× Master-Mix (Biolabs, England), 1μL each of 10μM forward and reverse primers (Inqaba-Biotec, South Africa), and 5μL of DNA template made up to 20μL with 3μL of DNAse/RNAse-free sterile water (BioConcept, USA) with the Master Cycler Nexus Gradient 230 (Eppendorf, Germany). .. Amplicons (10μL each) were run on 1.0% SafeView-Classic stained agarose gel at 80 V for 60 min, then viewed using the UV trans-illuminator E-BOX-CX5 TS imaging system (Vilber, France).



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    97
    Eppendorf AG master cycler nexus gradient 230
    Master Cycler Nexus Gradient 230, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/master+cycler+nexus+gradient+230/Mastercycler+nexus/pm40457246-69-57-62
    Average 97 stars, based on 1 article reviews
    master cycler nexus gradient 230 - by Bioz Stars, 2026-09
    97/100 stars
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